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■Bio-mathematics, Statistics and Nano-Technologies: Mosquito Control Strategies

main reason why we have kept them alive during the time of the preservation and the

transportation to the laboratory to proceed with further procedure and the identification

purposes. When mosquitos are collected with adults’ traps, they should be kept in small

container in thermobox with dried ice to preserve them before transporting to the lab.

4.14.2

Labeling

After the specimens have been put inside the vials (HLC collection) or inside small

containers (adults’ traps collection), every vial and small container should be labeled with

the date, locality or station, collection method used, and time of capturing to better analyze

them later.

4.14.3

Mosquito identification

The adults’ or larvae mosquito specimens, should be transported to the lab, before iden-

tification procedure. The identification of every specimen of mosquito caught, should be

carried out after every field trip using a stereomicroscope with light. Identification should

be performed based on adults’ or larva’s characters using established taxonomic keys (Reid

1968, Schaffner et al. 2001).

4.14.4

Dynamic and density of mosquito population

Mosquito dynamic has to do with the 24 hours’ activity and movement of the individu-

als of one population for food, water, shading, hiding places, breeding, laying eggs, taking

of a blood meal for female mosquito etc. Mosquito density has to do with the number of

mosquito individuals present and active in one area. The higher number of the individuals

in one population in one area, the higher will the mosquito density be.

4.15

DATA PROCESSING AND FIELD EVALUATION OF MOSQUITO BITES

VIA HLC METHOD AS A STANDARD TECHNIQUE FOR TESTING RE-

PELLENT TREATED IMPREGNATED TEXTILES

4.15.1

Calculation for the efficacy

Bites/man/hour formula should be used to calculate the biting activity cycles of fe-

male adult mosquitos. This formula gives in real time the exact number where a man can

get bitten from adult mosquitos in a specified time period of the day, in a specified site

and mosquito habitat. This does not give the efficacy, but gives us a clear overview on the

most anthropophilic mosquito species present, their biting activity cycle and their biting

behavior. After getting these results, it is easier to test the treated textiles or t-shirts with

a specific density and a specific or different repellent concentration on it, which will get

tested wash after wash, to see even the number of wash that the treated textile can handle

with still effective repellency feature.

Calculation for the efficacy tested on repellents impregnated textiles is performed by

the formula of R% below: